Human MIP3b ELISA Kit (CCL19) (ab100601)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2.05 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human MIP3b ELISA Kit (CCL19)
MIP-3 beta/CCL19 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
2.05 pg/ml - 500 pg/ml -
添加回収試験
78 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 71.8 65% - 89% Serum 84.43 75% - 94% Plasma 79.88 71% - 90% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s MIP3b Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3b in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MIP3b coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3b present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3b antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3b bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Human MIP3b 2 vials MIP3b Microplate (12 x 8 wells) 1 unit Recombinant Human MIP3b Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. -
組織特異性
Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Beta chemokine exodus 3
- Beta-chemokine exodus-3
- C C chemokine ligand 19
see all -
参照データベース
- Entrez Gene: 6363 Human
- Omim: 602227 Human
- SwissProt: Q99731 Human
- Unigene: 50002 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab100601 は 1 報の論文で使用されています。
- Ju Y et al. Loss of atypical chemokine receptor 4 facilitates C-C motif chemokine ligand 21-mediated tumor growth and invasion in nasopharyngeal carcinoma. Exp Ther Med 17:613-620 (2019). PubMed: 30651842