製品の概要

  • 製品名Human MIP3b ELISA Kit (CCL19)
    Macrophage Inflammatory Protein 3 beta キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    2.05 pg/ml - 500 pg/ml
  • 添加回収試験

    78 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 71.8 65% - 89%
    Serum 84.43 75% - 94%
    Plasma 79.88 71% - 90%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MIP3b Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3b in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP3b coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3b present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3b antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3b bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes.
  • 組織特異性Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Beta chemokine exodus 3
    • Beta-chemokine exodus-3
    • C C chemokine ligand 19
    • C-C motif chemokine 19
    • CC chemokine ligand 19
    • CCL 19
    • CCL19
    • CCL19_HUMAN
    • Chemokine (C C motif) ligand 19
    • Chemokine (CC motif) ligand 19
    • Chemokine C C Motif Ligand 19
    • Chemokine CC Motif Ligand 19
    • CK beta 11
    • CK beta-11
    • CKb 11
    • CKb11
    • EBI 1 ligand chemokine
    • EBI1 ligand chemokine
    • ELC
    • Epstein-Barr virus-induced molecule 1 ligand chemokine
    • Exodus 3
    • Exodus3
    • Macrophage inflammatory protein 3 beta
    • MGC34433
    • MIP 3 beta
    • MIP 3B
    • MIP-3-beta
    • MIP-3b
    • MIP3 beta
    • MIP3B
    • OTTHUMP00000000531
    • SCYA 19
    • SCYA19
    • Small inducible cytokine A19
    • Small inducible cytokine subfamily A (Cys Cys) member 1
    • Small-inducible cytokine A19
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MIP3b ELISA Kit (CCL19) 画像

  • Representative Standard Curve using ab100601.

プロトコール

Human MIP3b ELISA Kit (CCL19) (ab100601) 使用論文

ab100601 has not yet been referenced specifically in any publications.

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Thank you again for your email and for your patience while I have been in touch with the lab.



The lab has found that the standards are generally stable for short periods of time, but there isn't specific data regarding the standard...

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As you may know, this diluent does contain proteins and other solutes so it would not be uncommon to see some precipitation or cloudiness, especially if stored at low temperature and/or for a long period of ...

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Thank you for keeping me updated on the situation.

I've looked through our records and we haven't received reports from other users about high absorbance or kit-to-kit variability. I also looked through the orders sent to you, and all of the ...

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Thank you for your replies, and I'm glad to hear that the new kits have arrived.

All of the kits labelled "***"are from the same lot. The "-1, -2, -3" represent different assembly times for the kits. So, -1 kits were assem...

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Thanks for your reply and for keeping me updated.

I'll keep an eye out for your data, so you can send it whenever it's ready. I've asked the lab for some feedback on the issues you've noticed so far, and I'll get back to you once I hear from ...

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Thank you for keeping me updated, and I'm sorry to hear that the results haven't been as expected.

Do you have any data from these two partial runs that you could forward? I'm going to contact the lab to ask if they've noticed the precipitate...

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Thank you for your reply, and please let me know if you have any further questions or need anything else from me and I'll be happy to help.

Have a great day!

Thank you for contacting us with your questions, and for your patience while I've been in touch with the lab regarding this enquiry.

We also recommend starting with a 1:3 serum dilution, though the working dilution may need to be optimized fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"