製品の概要

  • 製品名Human MIP3a ELISA Kit (CCL20)
    Macrophage Inflammatory Protein 3 alpha キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1.5 pg/ml
  • 検出範囲
    0.8 pg/ml - 600 pg/ml
  • 添加回収試験

    94 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.25 83% - 102%
    Serum 95.39 83% - 103%
    Plasma 93.88 82% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MIP3a Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3a in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP3a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • 組織特異性Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Beta-chemokine exodus-1
    • C C motif chemokine ligand 20
    • C-C motif chemokine 20
    • CC chemokine LARC
    • Ccl20
    • CCL20(2-70)
    • CCL20_HUMAN
    • Chemokine (C C motif) ligand 20
    • Chemokine CC motif ligand 20
    • CKb4
    • Exodus
    • Exodus 1
    • LARC
    • Liver and activation-regulated chemokine
    • Macrophage inflammatory protein 3 alpha
    • MIP 3 alpha
    • MIP 3A
    • MIP-3-alpha
    • MIP-3a
    • MIP3A
    • SCYA20
    • Small inducible cytokine A20
    • Small inducible cytokine subfamily A (Cys Cys) member 20
    • Small-inducible cytokine A20
    • ST38
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MIP3a ELISA Kit (CCL20) 画像

  • Representative Standard Curve using ab100599.

  • Representative Standard Curve using ab100599.

プロトコール

Human MIP3a ELISA Kit (CCL20) (ab100599) 使用論文

ab100599 has not yet been referenced specifically in any publications.

Product Wall

CCL20-MIP3a secretion by human macrophages & DC

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL20-MIP3a human macrophage and DC secretion in culture for 3 and 7 days, isolated by negative selection.
The assay is easy to use and takes about 5.30hr.
Good specificity as shown by CCL3 secretion by M1 macrophages subtyes, particularly at day 3.
M0 = MCSF M1 = MCSF+IFNg M2a = MCSF+IL4 M2c = MCSF+TGFb or MCSF+IL10 or MCSF+Dexamethasone DC = GMCSF+IL4.
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投稿 May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"