製品の概要

  • 製品名Human MIP3 ELISA Kit (CCL23)
    Macrophage Inflammatory Protein 3 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 7 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 93.48 81% - 105%
    Serum 92.78 82% - 105%
    Plasma 94.16 83% - 106%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MIP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.
  • 組織特異性High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C-C motif chemokine 23
    • C6 beta chemokine
    • CCL 23
    • CCL23
    • CCL23(30-99)
    • CCL23_HUMAN
    • Chemokine CC motif ligand 23
    • ck beta 8
    • CK-beta-8
    • CKB 8
    • CKB-8
    • Ckb-8-1
    • CKB8
    • Hmrp-2a
    • Macrophage inflammatory protein 3
    • MIP 3
    • MIP-3
    • MIP3
    • MPIF 1
    • MPIF-1
    • MPIF1
    • Myeloid progenitor inhibitory factor 1
    • OTTHUMP00000163948
    • SCYA 23
    • SCYA23
    • Small inducible cytokine A 23
    • Small inducible cytokine subfamily A (Cys-Cys), member 23
    • Small inducible cytokine subfamily A, member 23
    • Small-inducible cytokine A23
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MIP3 ELISA Kit (CCL23) 画像

  • Representative Standard Curve using ab100611.

  • Representative Standard Curve using ab100611.

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Human MIP3 ELISA Kit (CCL23) (ab100611) 使用論文

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