Human MIP1 beta ELISA Kit (CCL4) (ab100597)
Key features and details
- Sensitivity: 2.5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human MIP1 beta ELISA Kit (CCL4)
CCL4/MIP-1 beta キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2.5 pg/ml -
検出範囲
4.1 pg/ml - 1000 pg/ml -
添加回収試験
95 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 97.42 85% - 104% Serum 95.64 82% - 102% Plasma 94.32 83% - 103% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s MIP1 beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP1 beta in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MIP1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MIP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
This product may also recognise CC4L [C-C motif chemokine 4-like], a family member which shares high homology with CCL4.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human MIP1 beta 2 vials MIP1 beta Microplate (12 x 8 wells) 1 unit Recombinant Human MIP1 beta Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all -
参照データベース
- Entrez Gene: 388372 Human
- Entrez Gene: 6351 Human
- Entrez Gene: 9560 Human
- Omim: 182284 Human
- Omim: 610757 Human
- Omim: 603782 Human
- SwissProt: Q8NHW4 Human
- SwissProt: P13236 Human
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab100597 は 2 報の論文で使用されています。
- Hosseinkhani B et al. (Sub)populations of extracellular vesicles released by TNF-a -triggered human endothelial cells promote vascular inflammation and monocyte migration. J Extracell Vesicles 9:1801153 (2020). PubMed: 32944190
- Hosseinkhani B et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). PubMed: 30131806