Human MIG ELISA Kit (CXCL9) ELISA Kit (ab100595)


  • 製品名
    Human MIG ELISA Kit (CXCL9) ELISA Kit
    CXCL9 キット 製品一覧
  • 検出方法
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    97 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 101.3 89% - 108%
    Serum 94.56 82% - 102%
    Plasma 96.32 84% - 103%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MIG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIG in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIG coated on a 96-well plate. Standards and samples are pipetted into the wells and MIG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム


  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human MIG (lyophilized) 2 vials
    MIG Microplate (12 x 8 wells) 1 unit
    Recombinant Human MIG Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 機能
    Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • 配列類似性
    Belongs to the intercrine alpha (chemokine CxC) family.
  • 細胞内局在
  • Information by UniProt
  • 別名
    • C-X-C motif chemokine 9
    • Chemokine (C-X-C motif) ligand 9
    • Chemokine, CXC motif, ligand 9
    • CMK
    • Crg 10
    • CXCL9
    • Gamma interferon induced monokine
    • Gamma-interferon-induced monokine
    • HuMIG
    • MIG
    • Monokine induced by gamma interferon
    • Monokine induced by interferon gamma
    • Monokine induced by interferon-gamma
    • SCYB9
    • Small inducible cytokine B9
    • Small inducible cytokine subfamily B member 9
    • Small-inducible cytokine B9
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab100595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100595.

  • Representative Standard Curve using ab100595.



ab100595 has not yet been referenced specifically in any publications.

レビューと Q&A

MIG-CXCL9 human macrophage secretion

Excellent Excellent 5/5 (Ease of Use)
MIG-CXCL9 secretion by human macrophages isolated by negative selection in culture for 3 & 7 days.
Kit easy to use, the assay takes about 6hr, good specificity as shown by higher secretion by M1 macrophages (MSCF+IFNg) compared to the others subtypes (M0=MCSF M2=MSCF+IL4 DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb)

Abcam user community

Verified customer

投稿 Apr 01 2014

Following up my e-mail from yesterday, the lab has requested some information that will help us assess the issue.

In addition to the raw data, could you please tell us:

At what temperature were the kits stored and for how long? Read More

Thank you for contacting us and bringing this to our attention. For our records, can you please tell me again the test you described that indicated the antibodies in these kits are likely not the issue? Have you tested any samples other than the standa...

Read More

Thank you for your call last week and for your patience while I have been intouch with the lab regarding your enquiry.

I've heard back from the lab, and there is no BSA or anything else in the wash buffer that we would expect to produce highe...

Read More