Human MER ELISA Kit (ab119604)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human MER ELISA Kit
MERTK キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 1 16 236pg/ml 14.63 6.2% 2 16 1665pg/ml 79.92 4.8% 3 16 4937pg/ml 197.48 4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 1 24 217pg/ml 13.67 6.2% 2 24 1701pg/ml 105.46 6.2% 3 24 4920pg/ml 221.4 4.5% -
サンプルの種類
Cell culture supernatant, Serum, Cell Lysate, Tissue Homogenate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
156 pg/ml - 10000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human MER in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MER in cell culture supernatants, cell lysates, tissue homogenates and serum.
A MER specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for MER is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MER amount of sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human MER antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human MER antibody 1 x 100µl Lyophilized recombinant Human MER standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究分野
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機能
In case of filovirus infection, seems to function as a cell entry factor. -
組織特異性
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
関連疾患
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
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別名
- c MER
- c mer proto oncogene tyrosine kinase
- c-mer
see all -
参照データベース
- Entrez Gene: 10461 Human
- Omim: 604705 Human
- SwissProt: Q12866 Human
- Unigene: 306178 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab119604 は 1 報の論文で使用されています。
- Hou X et al. The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis. Cell Rep 28:172-189.e7 (2019). PubMed: 31269438