Human MDC ELISA Kit (CCL22) (ab100591)

製品の概要

  • 製品名Human MDC ELISA Kit (CCL22)
    MDC キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1.5 pg/ml
  • 検出範囲
    1.65 pg/ml - 400 pg/ml
  • 添加回収試験

    82 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 83.18 74% - 98%
    Plasma 81.78 73% - 96%
    Cell culture extracts 82.65 73% - 97%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MDC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MDC in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MDC coated on a 96-well plate. Standards and samples are pipetted into the wells and MDC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MDC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MDC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
  • 組織特異性Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • A-152E5.1
    • ABCD 1
    • ABCD1
    • C-C motif chemokine 22
    • CC chemokine STCP-1
    • Ccl22
    • CCL22_HUMAN
    • Chemokine (C-C motif) ligand 22
    • DC/B-CK
    • Macrophage-derived chemokine
    • MDC(1-69)
    • MDC(7-69)
    • MGC34554
    • SCYA22
    • Small inducible cytokine A22
    • Small inducible cytokine subfamily A (Cys-Cys), member 22
    • Small-inducible cytokine A22
    • STCP 1
    • STCP1
    • Stimulated T cell chemotactic protein 1
    • Stimulated T-cell chemotactic protein 1
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MDC ELISA Kit (CCL22) 画像

  • Representative Standard Curve using ab100591.

  • Representative Standard Curve using ab100591.

  • Data shows secreation of MDC (CCL22) by human macrophages isolated by negative selection in culture for 3 days.

    Specificity shown by selective secretion by M2 macrophages (MSCF + IL4) compared to the others subtypes (M0 = MCSF, M1 = MSCF + IFNg, DEX = MCSF + Dexamethasone, IL10 = MCSF + IL10, TGFb = MCSF + TGFb).

プロトコール

Human MDC ELISA Kit (CCL22) (ab100591) 使用論文

ab100591 has not yet been referenced specifically in any publications.

Product Wall

CCL22-MDC secretion by Human macrophages

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL22-MDC secretion by human macrophages isolated by negative selection in culture for 3 days.
Kit easy to use, the assay takes about 5.30hr, excellent specificity as shown by selective secretion by M2 macrophages (MSCF+IL4) compared to the others subtypes (M0=MCSF M1=MSCF+IFNg DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb)
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投稿 Mar 12 2014

Thank you for contacting us.

We unfortunately do not have these kits available for human samples. I however have contacted the product manager and asked them if these products are in pipeline, I will get back to you once I get it confirmed. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"