製品の概要

  • 製品名Human MCP4 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 3 pg/ml
  • 検出範囲
    3.13 pg/ml - 200 pg/ml
  • 添加回収試験

    > 100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 105.8 74% - 133%
    Serum 139 128% - 149%
    Plasma 94.26 73% - 125%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MCP4 (monocyte chemotactic protein 4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MCP4 in serum, plasma, and cell culture supernatants. (Human MCP4 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human MCP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
  • 組織特異性Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾One major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C-C motif chemokine 13
    • CCL13
    • CCL13_HUMAN
    • Chemokine (C C motif) ligand 13
    • CK beta 10
    • CK-beta-10
    • CKb10
    • Macrophage/monocyte chemotactic protein 4
    • MCP-4
    • MGC17134
    • Monocyte chemoattractant protein 4
    • Monocyte chemotactic protein 4
    • NCC 1
    • NCC-1
    • NCC1
    • New CC chemokine 1
    • SCYA13
    • SCYL1
    • short chain
    • Small inducible cytokine A13
    • Small inducible cytokine A13 precursor
    • Small inducible cytokine subfamily A (Cys Cys) member 13
    • Small-inducible cytokine A13
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MCP4 ELISA Kit 画像

  • Data shows secretion of MCP4 by human macrophages isolated by negative selection in culture for 3 days.
    Specificity shown by selective secretion by M2 macrophages (MSCF + IL4) compared to the others subtypes (M0 = MCSF, M1 = MSCF + IFNg, DX = MCSF + Dexamethasone, IL10 = MCSF + IL10, TGFb = MCSF + TGFb).

  • Representative Standard Curve using ab100589.

  • Representative Standard Curve using ab100589.

プロトコール

Human MCP4 ELISA Kit (ab100589) 使用論文

ab100589 has not yet been referenced specifically in any publications.

Product Wall

CCL13 human macrophage secretion on day 3

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL13-MCP4 secretion by human macrophages isolated by negative selection in culture for 3 days.
Kit easy to use, the assay takes about 5.30hr, excellent specificity as shown by selective secretion by M2 macrophages (MSCF+IL4) compared to the others subtypes (M0=MCSF M1=MSCF+IFNg DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb)
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投稿 Mar 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"