製品の概要

  • 製品名
    Human MCP3 ELISA Kit
    MCP3 キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    24.58 pg/ml - 6000 pg/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 88.99 79% - 98%
    Plasma 95.19 88% - 106%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MCP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MCP3 in plasma, and cell culture supernatants. (Human MCP3 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human MCP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human MCP3 in normal serum with ab100588, only in serum spiked with Human MCP3.


    Get higher sensitivity in only 90 minutes with Human MCP3 ELISA Kit (CCL7) (ab193769) from our SimpleStep ELISA® range.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 機能
    Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
  • 配列類似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾
    O-glycosylated.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • C-C motif chemokine 7
    • Ccl7
    • CCL7_HUMAN
    • Chemokine CC Motif Ligand 7
    • Chemotactic protein 3
    • FIC
    • Intercrine/chemokine MARC
    • MARC
    • MCP 3
    • MCP-3
    • Monocyte chemoattractant protein 3
    • Monocyte chemotactic protein 3
    • NC28
    • RANTES/sis homolog
    • RP23-350G1.4
    • SCYA6
    • SCYA7
    • Small inducible cytokine A7
    • Small-inducible cytokine A7
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MCP3 ELISA Kit 画像

  • Representative Standard Curve Using ab100588.

  • Representative Standard Curve Using ab100588.

プロトコール

Human MCP3 ELISA Kit (ab100588) 使用論文

ab100588 has not yet been referenced specifically in any publications.

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Thank you for contacting us. Unfortunately the ELISA assays have not been used in conjunction with a DSX analyser (and we therefore do not have any specific protocols for this) but as long as the machine can accurately carry out the protocol as stated ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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