製品の概要

  • 製品名Human MCP2 ELISA Kit
    MCP2 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1.5 pg/ml
  • 検出範囲
    0.82 pg/ml - 200 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.25 85% - 105%
    Serum 93.88 84% - 104%
    Plasma 91.63 82% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MCP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP2 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MCP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MCP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
  • 組織特異性Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C C motif chemokine 8
    • CCL 8
    • Ccl8
    • CCL8_HUMAN
    • Chemokine (C C motif) ligand 8
    • Chemokine ligand 8
    • HC14
    • MCP 2
    • MCP-2
    • MCP-2(6-76)
    • Monocyte chemoattractant protein 2
    • Monocyte chemotactic protein 2
    • SCYA10
    • SCYA8
    • Small inducible cytokine A8
    • Small inducible cytokine subfamily A (Cys Cys) member 8
    • Small-inducible cytokine A8
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MCP2 ELISA Kit 画像

  • Representative Standard Curve using ab100587

  • Representative Standard Curve using ab100587

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Human MCP2 ELISA Kit (ab100587) 使用論文

ab100587 has not yet been referenced specifically in any publications.

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