Human MCP1 ELISA Kit (ab100586)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human MCP1 ELISA Kit
MCP1 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
2 pg/ml - 500 pg/ml -
添加回収試験
93 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 97.62 85% - 104% Serum 92.65 80% - 102% Plasma 90.42 79% - 101% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
製品の概要
Abcam’s MCP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human MCP1 ELISA Kit (CCL2) (ab179886) from our SimpleStep ELISA® range.
-
特記事項
Optimization may be required with urine samples.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Human MCP1 2 vials MCP1 Microplate (12 x 8 wells) 1 unit Recombinant Human MCP1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
機能
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- C-C motif chemokine 2
- CCL2
- CCL2_HUMAN
see all -
参照データベース
- Entrez Gene: 6347 Human
- Entrez Gene: 20296 Mouse
- Entrez Gene: 24770 Rat
- Omim: 158105 Human
- SwissProt: P13500 Human
- SwissProt: P10148 Mouse
- SwissProt: P14844 Rat
- Unigene: 303649 Human
see all
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (10)
ab100586 は 10 報の論文で使用されています。
- Cansby E et al. Silencing of STE20-type kinase STK25 in human aortic endothelial and smooth muscle cells is atheroprotective. Commun Biol 5:379 (2022). PubMed: 35440683
- Wang J et al. Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway. Exp Ther Med 19:1297-1303 (2020). PubMed: 32010302
- Santaella A et al. Cerebrospinal fluid monocyte chemoattractant protein 1 correlates with progression of Parkinson's disease. NPJ Parkinsons Dis 6:21 (2020). PubMed: 32944649
- Quan B et al. miR-141 alleviates LPS-induced inflammation injury in WI-38 fibroblasts by up-regulation of NOX2. Life Sci 216:271-278 (2019). PubMed: 30500550
- Ishii N et al. Conophylline suppresses pancreatic cancer desmoplasia and cancer-promoting cytokines produced by cancer-associated fibroblasts. Cancer Sci 110:334-344 (2019). PubMed: 30353606
- Nawaz SS et al. Potential role of serum fetuin-A in relation with pro-inflammatory, chemokine and adhesion molecules in diabetic kidney disease: a case-control study. Mol Biol Rep 46:1239-1246 (2019). PubMed: 30632070
- Meng D et al. Salvianolic acid B remits LPS-induced injury by up-regulating miR-142-3p in MH7A cells. Biomed Pharmacother 115:108876 (2019). PubMed: 31022596
- Qin D et al. Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells. Mol Med Rep 20:830-836 (2019). PubMed: 31180524
- Cao S et al. The upregulation of miR-101 promotes vascular endothelial cell apoptosis and suppresses cell migration in acute coronary syndrome by targeting CDH5. Int J Clin Exp Pathol 12:3320-3328 (2019). PubMed: 31934175
- Bai D et al. The effect of down-regulation of CCL5 on lipopolysaccharide-induced WI-38 fibroblast injury: a potential role for infantile pneumonia. Iran J Basic Med Sci 21:449-454 (2018). PubMed: 29922423