製品の概要

  • 製品名Human Mac-2BP ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 11.9%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.92 ng/ml
  • 検出範囲
    12.5 ng/ml - 200 ng/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 116 95% - 139%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Mac-2BP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Mac-2BP concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).

    Mac-2BP specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated Mac-2BP detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Mac-2BP amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
  • 組織特異性Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
  • 配列類似性Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 1 SRCR domain.
  • 細胞内局在Secreted. Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • 90K
    • Basement membrane autoantigen p105
    • BTBD17B
    • Galectin-3-binding protein
    • L3 antigen
    • Lectin galactoside-binding soluble 3-binding protein
    • LG3BP_HUMAN
    • LGALS3BP
    • MAC 2 BP
    • Mac-2 BP
    • Mac-2-binding protein
    • MAC2BP
    • Serum protein 90K
    • Tumor-associated antigen 90K
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Mac-2BP ELISA Kit 画像

  • Representative Standard Curve using ab119502.

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Human Mac-2BP ELISA Kit (ab119502) 使用論文

ab119502 has not yet been referenced specifically in any publications.

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