製品の概要

  • 製品名Human LYVE ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 8 pg/ml
  • 検出範囲
    8.19 pg/ml - 2000 pg/ml
  • 添加回収試験

    78 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 76 69% - 85%
    Serum 76.38 67% - 87%
    Plasma 79.07 68% - 86%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human LYVE ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LYVE in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human LYVE coated on a 96-well plate. Standards and samples are pipetted into the wells and LYVE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LYVE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LYVE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • 組織特異性Mainly expressed in endothelial cells lining lymphatic vessels.
  • 配列類似性Contains 1 Link domain.
  • 翻訳後修飾O-glycosylated.
  • 細胞内局在Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • 別名
    • cell surface retention sequence binding protein-1
    • Cell surface retention sequence-binding protein 1
    • CRSBP-1
    • CRSBP1
    • extracellular link domain containing 1
    • extracellular link domain-containing 1
    • Extracellular link domain-containing protein 1
    • HAR
    • Hyaluronic acid receptor
    • lymphatic vessel endothelial hyaluronan receptor 1
    • Lymphatic vessel endothelial hyaluronic acid receptor 1
    • LYVE-1
    • LYVE1
    • LYVE1_HUMAN
    • XLKD1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human LYVE ELISA Kit 画像

  • Representative Standard Curve using ab100585.

  • Representative Standard Curve using ab100585.

  • ab100585 was used to detect LYVE in Human heparin plasma samples according to the kit protocol. 10 µL sample was used per well.

    This image is courtesy of an anonymous Abreview

    See Abreview

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Human LYVE ELISA Kit (ab100585) 使用論文

ab100585 has not yet been referenced specifically in any publications.

Product Wall

Abreview for LYVE Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (Heparin plasma)
Specification: Heparin plasma
Amount of Sample: 10 uL µg
Type of Kit Used: ELISA
Positive control: Kit standards
Did you use the Abcam Kit protocol?: Yes

Additional data
Abcam response: all good, good images
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投稿 Oct 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"