製品の概要

  • 製品名
    Human Lysozyme ELISA Kit (LZM)
    Lysozyme キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.7%
  • サンプルの種類
    Milk
  • アッセイタイプ
    Competitive
  • 検出感度
    = 0.09 µg/ml
  • 検出範囲
    0.093 µg/ml - 6 µg/ml
  • 添加回収試験

    = 98 %

  • 全工程の試験時間
    3h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Lysozyme (LZM) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Lysozyme in milk.

    A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Lysozyme is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Lysozyme captured in plate.

  • アプリケーション
    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

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  • Representative Standard Curve using ab108877.

プロトコール

参考文献

ab108877 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your inquiry.
Unfortunately, we do not have any human lysozyme antibodies that have been used as a pair in a sandwich ELISA or for lateral flow available in our catalog.
I would like to recommend checking the Biocompare website ...

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Thank you for contacting us.

As suggested on the datasheet, I recommend diluting the samples 1:200 in Diluent. If necessary, further dilute samples within the range of 1:20 to 1:2000 into Diluent.

I hope this information is helpful...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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