Human Lysozyme ELISA Kit (LZM) (ab108877)
Key features and details
- Sensitivity: 0.09 µg/ml
- Range: 0.093 µg/ml - 6 µg/ml
- Sample type: Milk
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Human Lysozyme ELISA Kit (LZM)
Lysozyme キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 8.7% -
サンプルの種類
Milk -
アッセイタイプ
Competitive -
検出感度
= 0.09 µg/ml -
検出範囲
0.093 µg/ml - 6 µg/ml -
添加回収試験
= 98 %
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全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Lysozyme (LZM) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Lysozyme in milk.
A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Lysozyme is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Lysozyme captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml Biotinylated Human Lysozyme (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Lysozyme Microplate (12 x 8 well strips) 1 unit Lysozyme Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
関連疾患
Amyloidosis 8 -
配列類似性
Belongs to the glycosyl hydrolase 22 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- 1
- 1 4 beta N acetylmuramidase C
- 4-beta-N-acetylmuramidase C
see all -
参照データベース
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108877 は 1 報の論文で使用されています。
- Jackson BA et al. Human Milk Retains Important Immunologic Properties After Defatting. JPEN J Parenter Enteral Nutr 44:904-911 (2020). PubMed: 31599047