製品の概要

  • 製品名
    Human Lymphotactin ELISA Kit
    Lymphotactin キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 150 pg/ml
  • 検出範囲
    0.069 ng/ml - 50 ng/ml
  • 添加回収試験

    83 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 81.23 75% - 101%
    Serum 97.65 86% - 114%
    Plasma 72.19 68% - 109%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Lymphotactin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Lymphotactin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Lymphotactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Lymphotactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Lymphotactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lymphotactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human Lymphotactin 2 vials
    Lymphotactin Microplate (12 x 8 wells) 1 unit
    Recombinant Human Lymphotactin Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究分野
  • 機能
    Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
  • 組織特異性
    Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.
  • 配列類似性
    Belongs to the intercrine gamma family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • ATAC
    • C motif chemokine 1
    • Chemokine (C motif) ligand 1
    • Cytokine SCM-1
    • Cytokine SCM1
    • LPTN
    • LTN
    • Lymphotactin
    • Lymphotaxin
    • SCM 1
    • SCM 1 alpha
    • SCM-1-alpha
    • SCM1
    • SCM1 alpha
    • SCYC1
    • Small inducible cytokine C1
    • Small inducible cytokine subfamily C member 1
    • Small-inducible cytokine C1
    • XC chemokine ligand 1
    • Xcl1
    • XCL1_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab100584

  • Representative standard curve using ab100584

プロトコール

参考文献

ab100584 has not yet been referenced specifically in any publications.

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