Human lung tissue lysate (squamous cell carcinoma) - soluble fraction (male, 60 years) (ab43470)


  • 製品名Human lung tissue lysate (squamous cell carcinoma) - soluble fraction (male, 60 years)
    Lung ライゼート 製品一覧
  • 製品の詳細
    Human lung soluble fraction lysate
  • 特記事項Tissue: Lung Location: Left lower lobe. Diagnosis: Squamous cell carcinoma. Stage: IIIA T3N0M0 Grade: III Sex: Male Age: 60 years Gross findings: Tumor size 6 x 6 x 5 cm. Cut section hard, gray/white, and black. Regional lymph nodes cannot be assessed. Microscopic findings: Tumor composed of epithelial cells, coarse chromatin, bizarre nuclei, mitosis and large cytoplasm. Tumor necrosis; positive. Blood vessel invasion; negative. Lymphocytic response; positive. Soluble protein fraction.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43470: Extraction 1: soluble protein fraction ab43470 Human Lung Tumor Tissue lysate ab43469 Human Lung Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43472 Human Lung Tumor Tissue lysate ab43471 Human Lung Normal Tissue lysate (matched)


  • 製品の状態Liquid
  • 保存方法Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Constituents: PBS, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.0001% HEPES, 0.029% EDTA
  • Concentration information loading...
  • 特記事項(精製)Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究分野

    Human lung tissue lysate (squamous cell carcinoma) - soluble fraction (male, 60 years) (ab43470) 使用論文

    ab43470 has not yet been referenced specifically in any publications.

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    I have received the information about the tissue lysates that I was waiting for from the laboratory that produces these for us.

    The supplier of the lung lysates is confident that their measurements of protein concentration are correct, and s...

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    Thank you for the blot images and protocol detail. This makes clear what you described with regards to the differences in signal intensity among the samples, all loaded at 20ug of protein per lane based on the datasheet protein concentration specificat...

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