Human lung tissue lysate (squamous cell carcinoma) - insoluble fraction (male, 74 years) (ab42218)

製品の概要

  • 製品名Human lung tissue lysate (squamous cell carcinoma) - insoluble fraction (male, 74 years)
    Lung ライゼート 製品一覧
  • 製品の詳細
    Human lung insoluble fraction lysate
  • 特記事項

    Tissue: Lung Location: Left upper lobe. Diagnosis: Squamous cell carcinoma Stage: I T2N0M0 Grade: II Gross findings: Tumor size 7 cm diameter, well demarcated. Cut section soft, hemorrhagic and necrotic. Ipsilateral hilar lymph nodes show hyperplasia. Microscopic findings: Tumor shows proliferation of malignant epithelial cell clusters. Epithelial cells form clusters with irregular, basophilic, large nuclei and predominant nucleoli. Cytoplasm is abundant and clear. Nuclear chromatin is coarse and irregularly distributed. Mitotic figures are evident. The surrounding stroma is hemorrhagic and infiltrated by large numbers of lymphocytes, plasma cells, eosinophils and neutrophils. Tumor necrosis and blood vessel invasion are revealed.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42218: Extraction 1: soluble protein fraction ab42216 Human Lung Tumor Tissue lysate ab42215 Human Lung Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab42218 Human Lung Tumor Tissue lysate ab42217 Human Lung Normal Tissue lysate (matched)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 特記事項(精製)Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究分野

    Human lung tissue lysate (squamous cell carcinoma) - insoluble fraction (male, 74 years) (ab42218) 使用論文

    ab42218 has not yet been referenced specifically in any publications.

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