Human lung tissue lysate (large cell carcinoma) - insoluble fraction (male, 46 years) (ab42177)

製品の概要

  • 製品名Human lung tissue lysate (large cell carcinoma) - insoluble fraction (male, 46 years)
    Lung ライゼート 製品一覧
  • 製品の詳細
    Human lung insoluble fraction lysate
  • 特記事項

    Tissue: Lung Location: Left upper lobe. Diagnosis: Large cell carcinoma, well differentiated. Stage: III Grade: III Gross findings: Tumor size 6 x 5 cm diameter. Cut section hemorrhagic and dark red. Axillary lymph node: 1 Microscopic findings: Tumor shows malignant cells. The malignant cells are large and cuboidal or columnar. The cytoplasm is granular and vacuolated with large nucleoli. Nuclei have irregular nuclear membranes. Many monstrous nuclei and neutrophil cells. Axillary lymph node is positive.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42177: Extraction 1: soluble protein fraction ab42175 Human Lung Tumor Tissue lysate ab42174 Human Lung Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab42177 Human Lung Tumor Tissue lysate ab42176 Human Lung Normal Tissue lysate (matched)

製品の特性

  • 製品の状態Liquid
  • 保存方法Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 特記事項(精製)Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究分野

    Human lung tissue lysate (large cell carcinoma) - insoluble fraction (male, 46 years) (ab42177) 使用論文

    ab42177 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"