Human liver tissue lysate - total protein (ab29889)

製品の概要

  • 製品名Human liver tissue lysate - total protein
    Liver ライゼート 製品一覧
  • 製品の詳細
    Human liver total protein lysate
  • 特記事項


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • バッファーPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • ライゼート 備考Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab29889 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    Human liver tissue lysate - total protein 画像

    • All lanes : Anti-Selenium Binding Protein 1 antibody (ab90135)

      Lane 1 : Human liver tissue lysate - total protein (ab29889)
      Lane 2 : Human colon tissue lysate - total protein (ab30051)
      Lane 3 : Human spleen tissue lysate - total protein (ab29699)
      Lane 4 : Human placenta tissue lysate - total protein (ab29745)

      Lysates/proteins at 20 µg per lane.


      Exposure time : 5 seconds
    • All lanes : Anti-GAPDH (expected size 37kDa) at 1/10000 dilution

      Lane 1 : Human liver tissue lysate - total protein (ab29889) at 5 µg
      Lane 2 : Ladder
      Lane 3 : Human ES whole cell lysate at 10 µg

      Secondary
      Donkey Anti-Mouse IgG H&L (HRP) (ab205724) at 1/5000 dilution
      Developed using the ECL technique

      Exposure time : 5 seconds

      Data courtesy of an anonymous collaborator.

      Blocking buffer- 5% BSA /1% OvAlb /PBS (also works with 5% milk powder) for 3 hours.

    Human liver tissue lysate - total protein (ab29889) 使用論文

    This product has been referenced in:
    • Jaacks LM  et al. Placental expression of the heme transporter, feline leukemia virus subgroup C receptor, is related to maternal iron status in pregnant adolescents. J Nutr 141:1267-72 (2011). Read more (PubMed: 21593354) »

    See 1 Publication for this product

    Product Wall

    Thank you very much for your call yesterday and for your patience while I've looked into your enquiry.

    We have 5 liver lysates that are from different donors:

    Ab44563-

    http://www.abcam.com/Liver-Human-Tissue-Lysate-adult-n...

    Read More

    Thank you for calling Abcam today.

    I am sorry about the issues that you have been having with ab13868 in western blot. AS we discussed today, I am sending you a vial of human liver lysate to use as a positive control (although I am sending ab2...

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    Thank you for your enquiry

    We obtain this product from an external source and unfortunately they are not able to provide specific information about the sample preparation.

    I reviewed the literature and found the following protocol...

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    Thank you for your enquiry and your valuable feedback. We will change the shipping conditions for these products as soon as possible. This is to confirm that we will ship again one vial of each of these lysates as free of charge replacements - on d...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"