Human Lipocalin 2 ELISA Kit (NGAL) (ab113326)


  • 製品名Human Lipocalin 2 ELISA Kit (NGAL)
    Lipocalin-2 / NGAL キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 4 pg/ml
  • 検出範囲
    4.1 pg/ml - 1000 pg/ml
  • 添加回収試験

    96 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 75.47 69% - 88%
    Serum 103.9 94% - 112%
    Plasma 109.3 96% - 130%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Lipocalin 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Lipocalin 2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Lipocalin 2 coated on a 96- well plate. Standards and samples are pipetted into the wells and Lipocalin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Lipocalin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate


  • 機能Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • 組織特異性Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • 配列類似性Belongs to the calycin superfamily. Lipocalin family.
  • 細胞内局在Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • 別名
    • 24p3
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
    • HNL
    • Lcn2
    • Lipocalin 2
    • Lipocalin-2
    • Migration stimulating factor inhibitor
    • MSFI
    • Neutrophil gelatinase-associated lipocalin
    • NGAL
    • Oncogene 24p3
    • p25
    • Siderocalin
    • Siderocalin LCN2
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab113326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Lipocalin 2 ELISA Kit (NGAL) 画像

  • Representative Standard Curve using ab113326


Human Lipocalin 2 ELISA Kit (NGAL) (ab113326) 使用論文

ab113326 has not yet been referenced specifically in any publications.

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The shelf life of each of our kits is indicated in the individual protocol booklet.

ab99995 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80&d...

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Thank you for contacting us.
It is very important for this assay to dilute samples so that the measurements are in the range of the standard curve.
The datasheet indicates a range from 4.1 pg/ml - 1000 pg/ml (= 0.041- 1 ng/ml) for the NGAL kit...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these kits.
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I am sorry for the delay in getting back to you. I now have further information to share with you in regards to the Lipocalin-2 / NGAL Human ELISA Kit (ab113326).

I can confirm that we would recommend initially using the kit with serum or plas...

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I have received information regarding

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ab119596 KIM1 Mouse ELISA Kit
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ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
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To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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