製品の概要

  • 製品名Human LIMP II ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    10.24 pg/ml - 2500 pg/ml
  • 添加回収試験

    86 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.06 74% - 107%
    Serum 83 74% - 92%
    Plasma 79.65 71% - 86%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s LIMP II Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIMP-II in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human LIMP II coated on a 96-well plate. Standards and samples are pipetted into the wells and LIMP II present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIMP II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIMP II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Act as a lysosomal receptor for glucosylceramidase (GBA) targeting.
  • 関連疾患Defects in SCARB2 are the cause of progressive myoclonic epilepsy type 4 with or without renal failure (EPM4) [MIM:254900]. An autosomal recessive progressive myoclonic epilepsy associated with renal failure in some cases. Cognitive function is preserved. Myoclonus is a brief, involuntary twitching of a muscle or a group of muscles. Cognitive function is preserved.
    Note=Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
  • 配列類似性Belongs to the CD36 family.
  • 細胞内局在Lysosome membrane.
  • Information by UniProt
  • 別名
    • 85 kDa lysosomal membrane sialoglycoprotein
    • CD36
    • CD36 antigen-like 2
    • LGP85
    • LIMP II
    • Lysosome membrane protein 2
    • Lysosome membrane protein II
    • Scarb2
    • Scavenger receptor class B member 2
    • SCRB2_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human LIMP II ELISA Kit 画像

  • Representative Standard Curve using ab113336

プロトコール

Human LIMP II ELISA Kit (ab113336) 使用論文

ab113336 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113336.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"