製品の概要

  • 製品名Human LIGHT ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    96 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 96.25 86% - 105%
    Serum 95.58 84% - 103%
    Plasma 97.63 85% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s LIGHT Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIGHT in serum, plasma, and cell culture supernatants. (Human LIGHT concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human LIGHT coated on a 96-well plate. Standards and samples are pipetted into the wells and LIGHT present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIGHT antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIGHT bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
  • 組織特異性Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 翻訳後修飾N-glycosylated.
    The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
  • 細胞内局在Cytoplasm; Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • CD258
    • Delta transmembrane LIGHT
    • Herpes virus entry mediator ligand
    • Herpesvirus entry mediator A
    • Herpesvirus entry mediator ligand
    • HVEM L
    • HVEM-L
    • HVEML
    • Ligand for herpesvirus entry mediator
    • LTg
    • soluble form
    • TNF14_HUMAN
    • Tnfsf14
    • Tumor necrosis factor (ligand) superfamily member 14
    • Tumor necrosis factor ligand superfamily member 14
    • Tumor necrosis factor receptor like 2
    • Tumor necrosis factor superfamily member LIGHT
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human LIGHT ELISA Kit 画像

  • Representative Standard Curve using ab100583.

  • Representative Standard Curve using ab100583.

プロトコール

Human LIGHT ELISA Kit (ab100583) 使用論文

ab100583 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I can confirm that the ab100583 LIGHT Human ELISA Kit is QC tested with EDTA, heparin and citrate anti-coagulants so all 3 will be compatible.  Other anticoagulants have not been tested. I hope this will be helpful t...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"