Human Anti-Leishmania IgG ELISA Kit (ab108749)

製品の概要

  • 製品名Human Anti-Leishmania IgG ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Pos.Serum 7 7.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Pos.Serum 4 5.9%
  • テスト
    1 x 96 test
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプIndirect
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s anti-Leishmania IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Leishmania in Human serum and plasma.

     

    A 96-well plate has been precoated with Leishmania antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Leishmania-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leishmania IgG sample captured in plate.

  • アプリケーション適用あり: Indirect ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 保存方法Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Cover foil 1 unit
    IgG Sample Diluent Yellow, White cap 1 x 100ml
    Leishmania (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 1 unit
    Leishmania IgG Cut-off Control Yellow, Green cap 1 x 3ml
    Leishmania IgG Negative Control Yellow, Blue cap 1 x 2ml
    Leishmania IgG Positive Control Yellow, Red cap 1 x 2ml
    Leishmania Protein A Conjugate Blue, Black cap 1 x 20ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution Yellow cap 1 x 15ml
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab108749 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Indirect ELISA Use at an assay dependent dilution.

    プロトコール

    Human Anti-Leishmania IgG ELISA Kit (ab108749) 使用論文

    ab108749 has not yet been referenced specifically in any publications.

    Product Wall

    This product has been tested with L.infantum in human serum or plasma collected with citrate. Other Leishmania species hasn’t been tested so we are unsure how well this kit will perform. In case you are interested in buying and trying this produc...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"