製品の概要

  • 製品名Human LEC ELISA Kit (CCL16)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 8 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 96.47 85% - 104%
    Serum 95.29 84% - 103%
    Plasma 94.68 83% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s LEC (CCL16) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LEC in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human LEC coated on a 96-well plate. Standards and samples are pipetted into the wells and LEC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LEC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LEC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.
  • 組織特異性Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C-C motif chemokine 16
    • CCL16
    • CCL16_HUMAN
    • Chemokine CC-4
    • Chemokine LEC
    • CKb12
    • HCC-4
    • HCC4
    • IL-10-inducible chemokine
    • IL10 inducible chemokine
    • ILINCK
    • LCC-1
    • LCC1
    • Liver CC chemokine 1 precursor
    • Liver expressed chemokine
    • Liver-expressed chemokine
    • LMC
    • Lymphocyte and monocyte chemoattractant
    • Monotactin 1
    • Monotactin-1
    • MTN-1
    • Mtn1
    • NCC-4
    • NCC4
    • New CC chemokine 4
    • SCYA16
    • SCYL4
    • Small inducible cytokine A16 precursor
    • Small inducible cytokine subfamily A (Cys Cys) member 16
    • Small-inducible cytokine A16
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human LEC ELISA Kit (CCL16) 画像

  • Representative standard curve using ab100532

  • Representative standard curve using ab100532

プロトコール

Human LEC ELISA Kit (CCL16) (ab100532) 使用論文

ab100532 has not yet been referenced specifically in any publications.

Product Wall

LEC concentration in 16 human serum samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Human sera were diluted 10 times by Diluent A.
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投稿 Jun 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"