Human Laminin ELISA Kit (ab119599)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Laminin ELISA Kit
Laminin beta 2 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
156 pg/ml - 10000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Laminin in cell culture supernatants, serum and plasma (heparin, EDTA).
A Laminin specific rabbit polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from rabbit specific for Laminin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates ware washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Laminin amount of sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human Laminin antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-Human Laminin antibody 1 x 100µl 1 x 100µl Lyophilized Human Laminin standard 2 x 10ng 2 x 10ng Plate Seal 1 x 4 units 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究分野
- Information by UniProt
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別名
- Lamb2
- LAMB2_HUMAN
- Laminin B1s chain
see all -
参照データベース
- Entrez Gene: 3913 Human
- Omim: 150325 Human
- SwissProt: P55268 Human
- Unigene: 439726 Human
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab119599 は 3 報の論文で使用されています。
- Li H et al. Serum levels of laminin and von Willebrand factor in COVID-19 survivors 6 months after discharge. Int J Infect Dis 115:134-141 (2022). PubMed: 34843955
- Jung S et al. Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell. Int J Mol Sci 21:N/A (2020). PubMed: 32526920
- Narayanan K et al. Lineage-specific exosomes could override extracellular matrix mediated human mesenchymal stem cell differentiation. Biomaterials 182:312-322 (2018). PubMed: 30153612