製品の概要

  • 製品名Human Lactoferrin ELISA Kit (HLF2)
    Lactoferrin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.5%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.35 ng/ml
  • 検出範囲
    0.625 ng/ml - 40 ng/ml
  • 添加回収試験

    = 102 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Lactoferrin (HLF2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lactoferrin levels in Human cell culture supernatants, serum, urine, saliva, milk, cerebrospinal fluid and plasma.


    A Lactoferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lactoferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lactoferrin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Lactoferrin ELISA Kit (ab200015) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • 配列類似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • GIG12
    • Growth inhibiting protein 12
    • HLF2
    • Kaliocin 1
    • Lactoferrin
    • Lactoferroxin-C
    • Lactotransferrin
    • LF
    • LTF
    • Neutrophil lactoferrin
    • Talalactoferrin
    • TRFL_HUMAN
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Lactoferrin ELISA Kit (HLF2) 画像

  • Lactoferrin measured in biological fluids with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve Using ab108882.

プロトコール

Human Lactoferrin ELISA Kit (HLF2) (ab108882) 使用論文

This product has been referenced in:
  • Perrin MT  et al. A pilot study on the protein composition of induced nonpuerperal human milk. J Hum Lact 31:166-71 (2015). Sandwich ELISA ; Human . Read more (PubMed: 25288606) »
  • Gomes JA  et al. Brain iron metabolism and brain injury following subarachnoid hemorrhage: iCeFISH-pilot (CSF iron in SAH). Neurocrit Care 21:285-93 (2014). ELISA ; Human . Read more (PubMed: 24710655) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.



The concentration of standard in the lot you received is 40 ng/ml. The standard concentration is lot specific so be sure to check the vial each time you order a kit, or call us if the label is missing. Read More

Thanks for your enquiry.

I did some calculations and it seems if one starts with 5 ul milk sample and dilutes it in 6 successive 10-fold dilutions, where the final dilution is 50ul of a 100,000-fold dilution, there would be sufficient buffer ...

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Thank you for your interest. We do not have lactoferrin assay kits with longer shelf lives, but the 6 month expiration that is specifiedis very conservative. We are confidentthe kit will be effective, assuming proper storage, within that period.

Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in ...

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Thank you for contacting us.

The cross reactivity with bovine lactoferrin is less than 1%.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you very much for your email.

We have never tested equine samples. We have tested canine, bovine, monkey, mouse, rat, swine, and rabbit samples. See table below.

Species % Cross Reactivity
Canine 1%
Bovine 0.5%
...

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Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ort...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"