Human Kallikrein 1 ELISA Kit (KLK1) (ab119598)


  • 製品名
    Human Kallikrein 1 ELISA Kit (KLK1)
  • 検出方法
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Cell Lysate, Tissue Homogenate
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    156 pg/ml - 10000 pg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human KLK1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human KLK1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

    A KLK1 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KLK1 is then added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human KLK1 amount of sample captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム



  • 機能
    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
  • 組織特異性
    Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
  • 配列類似性
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾
    The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
  • Information by UniProt
  • 別名
    • Glandular kallikrein 1
    • hK1
    • Kallikrein 1 renal/pancreas/salivary
    • Kallikrein serine protease 1
    • Kallikrein-1
    • Kidney/pancreas/salivary gland kallikrein
    • KLK1
    • KLK1_HUMAN
    • Klk6
    • KLKR
    • Tissue kallikrein
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab119598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Kallikrein 1 ELISA Kit (KLK1) 画像

  • Representative Standard Curve using ab119598


Human Kallikrein 1 ELISA Kit (KLK1) (ab119598) 使用論文

ab119598 has not yet been referenced specifically in any publications.

Product Wall

Detection of human KLK1 in saliva is likely but unconfirmed by experiment. The antibodies in the assay should be capable of recognizing both isoforms 1 and 2, which are described at the UniProt entry at this link.

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This product will work for cell culture supernatants. Prepare samples as directed below: Cell culture supernates or tissue lysates: Remove particulates by centrifugation, assay immediately or aliquot and store samples at -20°C.

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We have never tested this ELISA kit with urine samples (only serum, plasma, and tissue extracts) so we do not know if it will work. Theoretically if urine has KLK1 concentrations within the range of detection 156 p...

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