製品の概要

  • 製品名Human IP-10 ELISA Kit (CXCL10)
    IP10 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 6 60.1pg/ml 0.9 1.6%
    B 6 32.4pg/ml 0.5 1.5%
    C 6 19.6pg/ml 0.3 1.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 18 56.9pg/ml 2.7 4.7%
    B 18 29.9pg/ml 2 6.5%
    C 18 17.7pg/ml 1.5 8.5%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 5.7 pg/ml
  • 検出範囲
    6.25 pg/ml - 200 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 120 118.7% - 122.4%

  • 全工程の試験時間
    3h 45m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IP-10 (CXCL10) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IP-10 in supernatants, buffered solutions, serum and plasma samples.

    A monoclonal antibody specific for IP-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IP-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IP-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IP-10 present in the samples. 

    This kit will recognize both endogenous and recombinant Human IP-10.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻訳後修飾CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Interferon gamma induced factor MOB1, mouse, homolog of
    • Interferon gamma induced protein 10
    • 10 kDa interferon gamma induced protein
    • 10 kDa interferon gamma-induced protein
    • C X C motif chemokine 10
    • C7
    • Chemokine (C X C motif) ligand 10
    • Chemokine CXC motif ligand 10
    • Crg 2
    • CRG2
    • CXCL10
    • CXCL10(1-73)
    • CXL10_HUMAN
    • Gamma IP10
    • Gamma-IP10
    • gIP 10
    • GIP10
    • IFI10
    • INP 10
    • INP10
    • Interferon activated gene 10
    • Interferon gamma induced cell line
    • Interferon inducible cytokine IP 10
    • Interferon inducible cytokine IP10
    • IP 10
    • IP-10
    • Mob 1
    • MOB1
    • Protein 10 from interferon (gamma) induced cell line
    • SCYB10
    • Small inducible cytokine B10
    • Small inducible cytokine B10 precursor
    • Small inducible cytokine subfamily B (Cys X Cys) member 10
    • Small inducible cytokine subfamily B CXC member 10
    • Small inducible cytokine subfamily B, member 10
    • Small-inducible cytokine B10
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab83700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IP-10 ELISA Kit (CXCL10) 画像

  • Representative Standard Curve using ab83700

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Human IP-10 ELISA Kit (CXCL10) (ab83700) 使用論文

ab83700 has not yet been referenced specifically in any publications.

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