Human Interferon Gamma + IL2 FLUOROSPOT Kit (without plates) (ab48451)

製品の概要

  • 製品名Human Interferon Gamma + IL2 FLUOROSPOT Kit (without plates)
    Interferon Gamma + IL2 キット 製品一覧
  • テスト
    5 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Cell culture extracts
  • アッセイタイプSandwich (qualitative)
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

     

    Principle

    After cell stimulation, locally produced cytokines are captured by IFN gamma and IL2 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti- IFN gamma FITC conjugated antibody and a biotinylated anti-IL2 antibody. Those are in turn recognised by anti-FITC green fluorescent dye and streptavidin-phycoerythrin conjugates. PVDF-bottomed-well plates are then read under a UV light beam. Green fluorescent spots indicate IFN gamma production while IL2 is revealed by red spots. Yellow spots will indicate dual cytokine producing cells.

  • アプリケーション適用あり: FluroSpotmore details
  • 試験プラットフォームReagents

製品の特性

  • 関連性IFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 細胞内局在Secreted
  • 別名
    • IFN gamma
    • IFNG
    • IL2
    • Immune interferon
    • Interferon gamma
    • Interleukin 2
    • Lymphokine
    • TCGF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab48451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
FluroSpot Use at an assay dependent dilution.

Human Interferon Gamma + IL2 FLUOROSPOT Kit (without plates) (ab48451) 使用論文

ab48451 has not yet been referenced specifically in any publications.

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1) Im IL2 ELISpot Kitab46546 sind nur die Reagenzien für den ELISpot enthalten, keine Platten. Den IL2 ELISpotKitMIT Platten haben wir alsab62927 erhältlich:

http://www.abcam.com/index.htm...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"