Human Insulin like Growth Factor 1 ELISA Kit (IGF1) (ab108873)

製品の概要

  • 製品名
    Human Insulin like Growth Factor 1 ELISA Kit (IGF1)
    IGF1 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.9%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    > 0.3 ng/ml
  • 検出範囲
    0.375 ng/ml - 24 ng/ml
  • 添加回収試験

    = 98 %

  • 全工程の試験時間
    5h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Insulin like Growth Factor 1 (IGF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin like Growth Factor 1 concentrations in Human cell culture supernatants, serum and plasma.


    An Insulin like Growth Factor 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Insulin like Growth Factor 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Insulin like Growth Factor 1 captured in plate.


    Get results in 90 minutes with Human IGF1 ELISA Kit (ab211651) from our SimpleStep ELISA® range.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Plasma/Serum Pretreatment Buffer 1 x 7.5ml
    20X Wash Buffer Concentrate 2 x 30ml
    70X Biotinylated Human Insulin like Growth Factor 1 Antibody 1 x 90µl
    Chromogen Substrate 1 x 8ml
    Insulin like Growth Factor 1 Microplate (12 x 8 well strips) 1 unit
    Insulin like Growth Factor 1 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野
  • 機能
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • 関連疾患
    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • 配列類似性
    Belongs to the insulin family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • IBP1
    • IGF I
    • IGF IA
    • IGF IB
    • IGF-I
    • Igf1
    • IGF1_HUMAN
    • IGF1A
    • IGFI
    • IGFIA
    • Insulin like growth factor 1
    • Insulin like growth factor 1 (somatomedin C)
    • Insulin like growth factor IA
    • Insulin like growth factor IB
    • Insulin-like growth factor I
    • Mechano growth factor
    • MGF
    • OTTHUMP00000195080
    • OTTHUMP00000195081
    • OTTHUMP00000195082
    • OTTHUMP00000195083
    • OTTHUMP00000195084
    • Somatomedia C
    • Somatomedin C
    • Somatomedin-C
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

画像

  • Representative Standard Curve using ab108873.

プロトコール

参考文献

ab108873 has not yet been referenced specifically in any publications.

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