Human IL-7 ELISA Set (without plates) (ab47357)
製品の概要
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製品名
Human IL-7 ELISA Set (without plates)
IL7 キット 製品一覧 -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 3 pg/ml -
検出範囲
6.25 pg/ml - 200 pg/ml -
全工程の試験時間
2h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-7 Human in vitro ELISA Set is designed for the quantitative measurement of IL-7 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human IL-7 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
These reagents are intended for research use only, not for diagnostic purposes.
The IL-7 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human IL-7.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 15 x 96 tests 5 x 96 tests 10 x 96 tests Chromogen TMB Substrate Solution 6 vials 2 vials 4 vials Detection biotinylated anti-IL-7 antibody 3 x 0.55ml 1 x 0.55ml 2 x 0.55ml IL-7 Capture antibody 3 x 0.25ml 1 x 0.25ml 2 x 0.25ml IL-7 standard Yellow 15 vials 5 vials 10 vials Streptavidin-HRP 3 vials 1 vial 2 vials -
研究分野
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関連性
Interleukin 7 (IL-7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL-7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. In myeloid lineage cells, IL-7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues. -
細胞内局在
Secreted -
別名
- IL 7
- IL7
- Interleukin 7
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参照データベース
- Entrez Gene: 3574 Human
- SwissProt: P13232 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47357の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. |
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab47357 は論文での使用が確認できていません。