Human IL6 ELISA Set (without plates) (ab48478)

製品の概要

  • 製品名
    Human IL6 ELISA Set (without plates)
    IL6 キット 製品一覧
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    200 pg/ml - 6.25 pg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-6 Human in vitro ELISA Set is designed for the quantitative measurement of IL-6 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The IL6 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA set is intended to measure human IL6 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Sensitivity: < 2 pg / ml

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Reagents

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-6 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-6 Capture antibody 1 x 0.25ml 2 x 0.25ml 3 x 0.25ml
    IL-6 Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials
    Ready-to-use TMB 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 x 25µl 2 x 25µl 3 x 25µl
  • 研究分野
  • 機能
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 関連疾患
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 配列類似性
    Belongs to the IL-6 superfamily.
  • 翻訳後修飾
    N- and O-glycosylated.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Hepatocyte stimulating factor
    • HSF
    • Hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta-2
    • Interleukin 6
    • Interleukin-6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab48478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA
  • 追加情報
    ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 画像

    • IL-6 Standard curve ranging from 6.25 to 200 pg/mL

    参考文献

    ab48478 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for your enquiry. I apologise for the delay in obtaining this information for you. We have been waiting for confirmation from our source.

    I can confirm the reagents and materials required but not provided with ab48478 IL6 Human Eli...

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    Thank you for contacting us. I apologise for the delay.
    Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
    The only problem I would suspect is the high conc...

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    Thank you for contacting us.

    1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) an...

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    Thank you for your reply.

    I can suggest the same would apply for ab48478. I can recommend to purchase ELISA plates specifically. to ensure they are of the higher quality required.

    I hope this will be helpful. If you have any further...

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    Thank you for contacting us. Overnight Incubation of plate at 4C is sort of general recommendation we provide to our customers. 3-4 hours at 4C can also be tried; the assay should be OK However in case of no readings we would be suggesting the over...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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