Human IL4 ELISA Set (without plates) (ab48474)

製品の概要

  • 製品名Human IL4 ELISA Set (without plates)
    IL4 キット 製品一覧
  • テスト
    5 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Other biological fluids
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.5 pg/ml
  • 検出範囲
    35 pg/ml - 1.1 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-4 Human in vitro ELISA Set is designed for the quantitative measurement of IL-4 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The IL4 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA set is intended to measure human IL4 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Recognizes both natural and recombinant human IL-4 Sensitivity: < 0.7 pg / ml

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームReagents

製品の特性

  • 関連性IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
  • 細胞内局在Secreted
  • 別名
    • B cell stimulatory factor 1
    • Binetrakin
    • BSF1
    • Interleukin 4
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab48474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA
  • 追加情報ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Human IL4 ELISA Set (without plates) 画像

    • IL-4 Standard curve ranging from 1.1 to 35 pg/mL

    Human IL4 ELISA Set (without plates) (ab48474) 使用論文

    ab48474 has not yet been referenced specifically in any publications.

    Product Wall

    Unfortunately, the specific antibody pair in ab48474 is not available for sale separately. However, we do have 2 anti-IL4 primary antibodies that have been tested and are guaranteed to detect IL4 in a sandwich ELISA format for 12 months after purchase....

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    The lab recommends the following for samples human serum or plasmas : 1/2 (50µl sample + 50µl diluent) and multiply the calculated concentration by 2. Regarding cell supernatants, expected values are very dependent to the cells assa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"