製品の概要

  • 製品名
    Human IL17 ELISA Set (without plates)
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 3 pg/ml
  • 検出範囲
    3.125 pg/ml - 100 pg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-17 Human in vitro ELISA Set is designed for the quantitative measurement of IL-17 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The IL-17A ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure human IL-17A in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    SPECIFICITY: Recognizes both natural and recombinant human IL-17A

    RANGE: 3.12pg/ml - 100pg/ml

    SENSITIVITY: <2.3pg/ml

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Reagents

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 5 x 96 tests 10 x 96 tests
    Detection biotinylated anti-IL-17 antibody 1 x 0.55ml 2 x 0.55ml
    IL17 Capture Antibody 1 x 0.5ml 2 x 0.5ml
    IL17 standard 5 vials 10 vials
    Ready-to-use TMB 2 vials 4 vials
    Streptavidin-HRP 1 vial 2 vials
  • 研究分野
  • 関連性
    IL17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6, IL8 and GCSF production in fibroblasts; the enhancement of surface expression of ICAM 1 in fibroblasts; activation of NF kappaB and costimulation of T cell proliferation. IL17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL17 consists of 155 amino acids. To generate the mature IL17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL17 shows approximately 62.5% amino acid homology to mouse IL17 and 58% amino acid homology to rat IL17.
  • 細胞内局在
    Secreted
  • 別名
    • CTLA 8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • IL 17A
    • IL17A
    • Interleukin 17A
    • Interleukin17
    • Interleukin17A
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab83707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • IL-17 Standard curve ranging from 3.125 to 100 pg/mL

参考文献

ab83707 has not yet been referenced specifically in any publications.

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