製品の概要

  • 製品名
    Human IL13 ELISPOT Kit
    IL13 キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Cell culture extracts
  • アッセイタイプ
    Sandwich (qualitative)
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    Principal:

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

     

    Recognizes both natural and recombinant human IL13

  • アプリケーション
    適用あり: ELISpotmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests 5 x 96 tests
    Bovine Serum Albumin 1 x 200mg 1 x 1g
    Human IL13 Pre-coated 96-well PDVF-bottomed plates 1 unit 5 units
    Clone B-P6 Biotinylated detection antibody, 1 vial 1 vial
    Ready-to-use BCIP/NBT substrate buffer 1 x 10ml 1 x 50ml
    Streptavidin - Alkaline Phosphatase conjugated 1 x 10µl 1 x 50µl
  • 研究分野
  • 関連性
    IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL13 down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. It is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. IL13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
  • 細胞内局在
    Secreted
  • 別名
    • ALRH
    • BHR1
    • IL 13
    • Interleukin 13
    • NC30
    • P600
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab62941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISpot Use at an assay dependent dilution.

参考文献

ab62941 has not yet been referenced specifically in any publications.

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