製品の概要

  • 製品名
    Human IL10 + IL2 Human ELISPOT Kit
    IL10 + IL2 キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Cell culture extracts
  • アッセイタイプ
    Sandwich (qualitative)
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    The dual colour Elispot allows you to monitor the production of two cytokines simultaneously in the same well.

  • アプリケーション
    適用あり: ELISpotmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 5 x 96 tests 10 x 96 tests
    10 x concentrate buffer for the preparation of AEC buffer 1 x 5ml 2 x 5ml
    50 x concentrate AEC substrate buffer 1 x 1ml 2 x 1ml
    96 PVDF-bottomed-well plates. 5 units 10 units
    Anti-FITC antibody HRP conjugate 1 x 100µl 2 x 100µl
    Bovine Serum Albumin 1 x 1g 2 x 1g
    Human IL-10 Capture antibody 1 x 500µl 2 x 500µl
    Human IL-2 Capture antibody 1 x 500µl 2 x 500µl
    IL10 FITC conjugated detection antibody 1 vial 2 vials
    IL-2 Biotinylated detection antibody 1 vial 2 vials
    Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml
    Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl
  • 研究分野
  • 関連性
    IL2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. IL10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 細胞内局在
    Secreted
  • 別名
    • Aldesleukin
    • CSIF
    • Cytokine synthesis inhibitory factor
    • Interleukin 10
    • Interleukin 2
    • Lymphokine
    • T cell growth factor
    • TCGF
    • TGIF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab48456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISpot Use at an assay dependent dilution.

参考文献

ab48456 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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