Human IL10 + IL2 ELISPOT Kit (without plates) (ab48455)


  • 製品名Human IL10 + IL2 ELISPOT Kit (without plates)
    IL10 + IL2 キット 製品一覧
  • テスト
    5 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Cell culture extracts
  • アッセイタイプSandwich (qualitative)
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    The dual colour Elispot allows you to monitor the production of two cytokines simultaneously in the same well.



    After cell stimulation, locally produced cytokines are captured by IL10 and IL2 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti-IL10 FITC conjugated antibody and a biotinylated anti-IL2 antibody. Those are in turn recognised by anti-FITC HRP and streptavidin-AP conjugates. PVDF-bottomed-well plates are then incubated first with AEC substrate buffer, washed and subsequently incubated with BCIP/NBT. Coloured red/brownish spots indicate IL10 production while IL2 is revealed by blue/purple spots.

  • アプリケーション適用あり: ELISpotmore details
  • 試験プラットフォームReagents


  • 保存方法Store at +4°C. Please refer to protocols.
  • 内容 5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests
    10 x concentrate buffer for the preparation of AEC buffer 1 x 5ml 2 x 5ml 3 x 5ml 4 x 5ml
    50 x concentrate AEC substrate buffer 1 x 1ml 2 x 1ml 3 x 1ml 4 x 1ml
    Anti-FITC antibody HRP conjugate 1 x 100µl 2 x 100µl 3 x 100µl 4 x 100µl
    Bovine Serum albumin 1 x 1g 2 x 1g 3 x 1g 4 x 1g
    Human IL-10 Capture antibody 1 x 500µl 2 x 500µl 3 x 500µl 4 x 500µl
    Human IL-2 Capture antibody 1 x 500µl 2 x 500µl 3 x 500µl 4 x 500µl
    IL10 FITC conjugated detection antibody 1 vial 2 vials 3 vials 4 vials
    IL-2 Biotinylated detection antibody 1 vial 2 vials 3 vials 4 vials
    Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml 3 x 50ml 4 x 50ml
    Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl 3 x 50µl 4 x 50µl
  • 研究分野
  • 関連性IL2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. IL10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 細胞内局在Secreted
  • 別名
    • Aldesleukin
    • CSIF
    • Cytokine synthesis inhibitory factor
    • Interleukin 10
    • Interleukin 2
    • Lymphokine
    • T cell growth factor
    • TCGF
    • TGIF
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab48455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISpot Use at an assay dependent dilution.

Human IL10 + IL2 ELISPOT Kit (without plates) (ab48455) 使用論文

ab48455 has not yet been referenced specifically in any publications.

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