製品の概要

  • 製品名Human IL-9 ELISA Kit (Interleukin-9)
    IL9 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.1 ng/ml
  • 検出範囲
    0.04 ng/ml - 30 ng/ml
  • 添加回収試験

    98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 102.1 90% - 110%
    Serum 101.6 95% - 110%
    Plasma 82.4 67% - 98%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-9 (Interleukin-9) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-9 in serum, plasma and cell culture supernatants. (Human IL-9 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human IL-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Supports IL-2 independent and IL-4 independent growth of helper T-cells.
  • 配列類似性Belongs to the IL-7/IL-9 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Cytokine P40
    • Homolog of mouse T cell and mast cell growth factor 40
    • HP40
    • IL-9
    • Il9
    • IL9_HUMAN
    • Interleukin 9
    • Interleukin-9
    • Mast cell growth factor
    • MCGF
    • Megakaryoblast growth factor
    • p40 cytokine
    • T cell growth factor 3
    • T cell growth factor p40
    • T-cell growth factor P40
    • TCGF 3
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-9 ELISA Kit (Interleukin-9) 画像

  • Representative standard curve using ab100577

  • Representative standard curve using ab100577

プロトコール

Human IL-9 ELISA Kit (Interleukin-9) (ab100577) 使用論文

ab100577 has not yet been referenced specifically in any publications.

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