サンプル | N | 平均値 | SD | CV% |
---|---|---|---|---|
Overall | 4.4% |
サンプル | N | 平均値 | SD | CV% |
---|---|---|---|---|
Sample 1 | 18 | 25pg/ml | 3 | 12.2% |
Sample 2 | 18 | 12pg/ml | 1 | 8.7% |
Sample 3 | 18 | 6pg/ml | 1 | 13.7% |
Sample 4 | 18 | 41pg/ml | 2 | 5.9% |
Sample 5 | 18 | 31pg/ml | 1 | 4.9% |
93
%
Abcam’s IL-6 (Interleukin-6) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognise both natural and recombinant Human IL-6.
A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-6.
Get results in 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.
内容 | ラベル | 1 x 96 tests | 2 x 96 tests |
---|---|---|---|
10X Standard Diluent Buffer | Black | 1 x 25ml | 1 x 25ml |
200X Wash Buffer | White | 1 x 10ml | 2 x 10ml |
Plastic Plate Covers | 1 x 2 units | 1 x 4 units | |
Biotinylated Antibody Diluent | Red | 1 x 7ml | 1 x 13ml |
Biotinylated anti-IL-6 | Red | 1 x 0.4ml | 2 x 0.4ml |
Chromogen TMB Substrate Solution | 1 x 11ml | 1 x 24ml | |
HRP Diluent | Red | 1 x 23ml | 1 x 23ml |
IL-6 Control | Silver | 2 vials | 4 vials |
IL-6 Microplate (12 x 8 well strips) | 1 unit | 2 units | |
IL-6 Standard (Lyophilized) | Yellow | 2 vials | 4 vials |
Standard Diluent (Human Serum) | 1 x 7ml | 2 x 7ml | |
Stop Reagent | Black | 1 x 11ml | 2 x 11ml |
Streptavidin-HRP | 2 x 5µl | 4 x 5µl |
Our Abpromise guarantee covers the use of ab46042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
Representative Standard Curve using ab46042
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"