Human IL-6 ELISA Kit (Interleukin-6) High Sensitivity (ab46042)
製品の概要
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製品名Human IL-6 ELISA Kit (Interleukin-6) High Sensitivity
IL6 キット 製品一覧 -
検出方法Colorimetric
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再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Sample 1 18 25pg/ml 3 12.2% Sample 2 18 12pg/ml 1 8.7% Sample 3 18 6pg/ml 1 13.7% Sample 4 18 41pg/ml 2 5.9% Sample 5 18 31pg/ml 1 4.9% -
サンプルの種類Cell culture supernatant, Serum, Plasma
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アッセイタイプSandwich (quantitative)
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検出感度< 0.8 pg/ml
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検出範囲1.56 pg/ml - 50 pg/ml
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添加回収試験
93 %
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全工程の試験時間3h 45m
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ステップMultiple steps standard assay
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種交差性交差種: Human
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製品の概要
Abcam’s IL-6 (Interleukin-6) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognise both natural and recombinant Human IL-6.
A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-6.
Get results in 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.
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アプリケーション適用あり: Sandwich ELISAmore details
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試験プラットフォームMicroplate
法規制情報
製品の特性
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保存方法Store at +4°C. Please refer to protocols.
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内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Plastic Plate Covers 1 x 2 units 1 x 4 units Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-IL-6 Red 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 23ml IL-6 Control Silver 2 vials 4 vials IL-6 Microplate (12 x 8 well strips) 1 unit 2 units IL-6 Standard (Lyophilized) Yellow 2 vials 4 vials Standard Diluent (Human Serum) 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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機能Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
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関連疾患Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性Belongs to the IL-6 superfamily.
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翻訳後修飾N- and O-glycosylated.
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細胞内局在Secreted.
- Information by UniProt
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別名
- B cell differentiation factor
- B cell stimulatory factor 2
- B-cell stimulatory factor 2
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参照データベース
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
関連製品
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SimpleStep ELISA kits
アプリケーション
Our Abpromise guarantee covers the use of ab46042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
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Sandwich ELISA - IL-6 (Interleukin-6) High Sensitivity Human ELISA Kit (ab46042)IL-6 was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 �g x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) and LPS (background signal subtracted) (duplicates +/- SD). -
Typical Standard CurveRepresentative Standard Curve using ab46042
プロトコール
参考文献
This product has been referenced in:
- Zhao C et al. Long-term bicycle riding ameliorates the depression of the patients undergoing hemodialysis by affecting the levels of interleukin-6 and interleukin-18. Neuropsychiatr Dis Treat 13:91-100 (2017). ELISA ; Human . Read more (PubMed: 28096677) »
- Sun L et al. Actinidia chinensis Planch. Improves the Indices of Antioxidant and Anti-Inflammation Status of Type 2 Diabetes Mellitus by Activating Keap1 and Nrf2 via the Upregulation of MicroRNA-424. Oxid Med Cell Longev 2017:7038789 (2017). Human . Read more (PubMed: 28642811) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"