Human IL-6 ELISA Kit (Interleukin-6) ELISA Kit (ab108867)

製品の概要

  • 製品名Human IL-6 ELISA Kit (Interleukin-6) ELISA Kit
    IL6 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.4%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    > 0.008 ng/ml
  • 検出範囲
    0.008 ng/ml - 0.5 ng/ml
  • 添加回収試験

    99 %

  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-6 (Interleukin-6) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 concentrations in Human cell culture supernatants, serum and plasma.

    An IL-6 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-6 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-6 captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 関連疾患Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 配列類似性Belongs to the IL-6 superfamily.
  • 翻訳後修飾N- and O-glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • B cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta-2
    • Interleukin 6
    • Interleukin BSF2
    • Interleukin-6
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human IL-6 ELISA Kit (Interleukin-6) ELISA Kit 画像

  • Representative Standard Curve Using ab108867.

プロトコール

Human IL-6 ELISA Kit (Interleukin-6) ELISA Kit (ab108867) 使用論文

ab108867 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.
Do you have the catalogue numbers of the kits you're inquiring about? We have 7 different IL6 Human ELISA kits in the catalogue: ab46044, ab46042, ab46027, ab46041, ab100572, ab100573 and ab108867.
The IL6 High Sen...

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Thank you for contacting us. I apologise for the delay.
Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
The only problem I would suspect is the high conc...

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Thank you for contacting us.

1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"