製品の概要

  • 製品名Human IL-5 ELISA Kit (Interleukin-5)
    IL5 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.83 84% - 103%
    Serum 95.46 83% - 103%
    Plasma 96.32 84% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-5 (Interleukin-5) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • 配列類似性Belongs to the IL-5 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • B cell differentiation factor I
    • B-cell differentiation factor I
    • Colony stimulating factor eosinophil
    • EDF
    • Eosinophil differentiation factor
    • IL 5
    • IL-5
    • IL5
    • IL5_HUMAN
    • Interleukin 5
    • Interleukin 5 (colony stimulating factor eosinophil)
    • Interleukin-5
    • Interleukin5
    • T cell replacing factor
    • T-cell replacing factor
    • TRF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-5 ELISA Kit (Interleukin-5) 画像

  • Representative standard curve using ab100571

  • Representative standard curve using ab100571

プロトコール

Human IL-5 ELISA Kit (Interleukin-5) (ab100571) 使用論文

ab100571 has not yet been referenced specifically in any publications.

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