製品の概要

  • 製品名
    Human IL-4 Receptor ELISA Kit (CD124)
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 563.7pg/ml 17.8 3.1%
    Sample B 8 140.6pg/ml 9.6 6.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 530.5pg/ml 35.1 6.6%
    Sample B 8 106.5pg/ml 7.58 7.1%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 11.5 pg/ml
  • 検出範囲
    31.25 pg/ml - 1000 pg/ml
  • 全工程の試験時間
    1h 45m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human IL-4 Receptor (IL-4R) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-4 receptor molecule (CD124) in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4R present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-4R.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-4R Red 1 x 0.3ml 2 x 0.3ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-4R Microplate (12 x 8 well strips) 1 unit 2 units
    IL-4R Standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 関連性
    IL4R binds interleukin 4 and interleukin 13 to regulate IgE production. It also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the IL4R can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene.
  • 細胞内局在
    Cell membrane; single-pass type I membrane protein. Isoform 2: Secreted protein
  • 別名
    • IL 4R alpha
    • IL4RA
    • ILR4A
    • Interleukin 4 receptor
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab46022

プロトコール

参考文献

ab46022 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab46022.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録