Human IL-4 ELISA Kit (Interleukin-4) High Sensitivity (ab46063)

製品の概要

  • 製品名
    Human IL-4 ELISA Kit (Interleukin-4) High Sensitivity
    IL4 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 8 7.43pg/ml 0.9 12.3%
    B 8 6.2pg/ml 0.7 10.8%
    C 8 8.77pg/ml 0.7 7.6%
    D 8 7.36pg/ml 0.4 6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 9 7.1pg/ml 0.83 11.8%
    B 9 6.47pg/ml 0.72 11.1%
    C 9 8.76pg/ml 1.37 15.7%
    D 9 7.44pg/ml 1.2 16.1%
    E 7 2pg/ml 0.874 17%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.31 pg/ml
  • 検出範囲
    0.31 pg/ml - 10 pg/ml
  • 添加回収試験

    101 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95 97% - 127%
    Serum 69 61% - 75%

  • 全工程の試験時間
    4h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-4 (Interleukin-4) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in Human serum, plasma, buffered solution and cell culture media.

    A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-4.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml
    200X Wash Buffer White 1 x 10ml
    Amplification Diluent Brown & blue spot 1 x 25ml
    Amplifier Yellow 1 x 200µl
    Biotinylated anti IL-4 1 x 400µl
    Biotinylated Antibody Diluent Red 1 x 7.5ml
    Chromogen TMB Substrate Solution 1 x 11ml
    HRP Diluent Red 1 x 25ml
    IL-4 Microplate (12 x 8 well strips) 1 unit
    IL-4 Standard (Lyophilized) 2 vials
    Stop Reagent Black 1 x 11ml
    Streptavidin-HRP 2 x 5µl
  • 研究分野
  • 関連性
    IL4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL4 encoding distinct isoforms have been reported.
  • 細胞内局在
    Secreted
  • 別名
    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF1
    • IL 4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Standard curve of human IL-4, with background signal subtracted (duplicates; +/- SD).

  • IL-4 measured in cell culture supernatants from control (PBMC) or treated (PBMC+PHA; 2% PHA-M; LifeTechnologies) cells, grown for 48 hours (duplicates +/- SD). Samples tested neat - 1/10, control supernatant gave signals below level of detection.

  •  Representative Standard Curve using ab46063

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参考文献

ab46063 has not yet been referenced specifically in any publications.

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