製品の概要

  • 製品名Human IL-33 ELISA Kit (Interleukin-33)
    IL33 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 6.9%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.2 pg/ml
  • 検出範囲
    7.8 pg/ml - 500 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 73% - 92%
    Serum 67% - 79%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-33 (Interleukin-33) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-33 in cell culture supernatant, serum, and plasma (citrate, heparin).

    IL-33 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human IL-33 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-33 captured on the plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines.
  • 組織特異性Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • 配列類似性Belongs to the IL-1 family. Highly divergent.
  • 翻訳後修飾Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C9orf26
    • DVS27
    • DVS27 related protein
    • IL-1F11
    • IL-33
    • IL1F11
    • IL33
    • IL33_HUMAN
    • Interleukin-1 family member 11
    • Interleukin-33
    • NF-HEV
    • NFEHEV
    • Nuclear factor from high endothelial venules
    • RP11-575C20.2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-33 ELISA Kit (Interleukin-33) 画像

  • Representative Standard Curve using ab119547.

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Human IL-33 ELISA Kit (Interleukin-33) (ab119547) 使用論文

ab119547 has not yet been referenced specifically in any publications.

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