Human IL-31 ELISA Kit (Interleukin-31) (ab119546)

製品の概要

  • 製品名Human IL-31 ELISA Kit (Interleukin-31)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 2.5%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    1 pg/ml
  • 検出範囲
    7.8 pg/ml - 500 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 115% - 123%
    Serum 86% - 106%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-31 (Interleukin-31) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-31 in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    IL-31 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an Biotin-conjugated anti-Human IL-31 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-31 captured on the plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
  • 組織特異性Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • IL-31
    • IL31
    • IL31_HUMAN
    • Interleukin-31
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-31 ELISA Kit (Interleukin-31) 画像

  • Representative Standard Curve using ab119546.

プロトコール

Human IL-31 ELISA Kit (Interleukin-31) (ab119546) 使用論文

ab119546 has not yet been referenced specifically in any publications.

Product Wall

very good Elisa

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We obtain reliable and reproducible results with this Elisa.
The protocol booklet is well explained, easy to understand and easy to perform.
I used this kit for serum samples from a comparison of healthy donor and atopic patients and and cell culture supernatants.
The kit is easy to use and quick compared to other Sandwich Elisa.
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投稿 Apr 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"