Human IL-29 ELISA Kit (ab100568)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-29 ELISA Kit
IL-29 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
13.72 pg/ml - 10000 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 124 104% - 143% Serum 97.78 87% - 105% Plasma 112.7 95% - 145% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-29 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-29 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-29 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-29 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-29 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-29 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human IL-29 2 vials IL-29 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-29 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine with immunomodulatory activity. May play a role in antiviral immunity. Up-regulates MHC class I antigen expression. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. -
配列類似性
Belongs to the IL-28/IL-29 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Cytokine ZCYTO21
- IFN lambda 1
- IFN-lambda-1
see all -
参照データベース
- Entrez Gene: 282618 Human
- Omim: 607403 Human
- SwissProt: Q8IU54 Human
- Unigene: 406745 Human
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab100568 は論文での使用が確認できていません。