Human IL-28A ELISA Kit (Interleukin-28A) (ab100567)

製品の概要

  • 製品名
    Human IL-28A ELISA Kit (Interleukin-28A)
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    13.72 pg/ml - 10000 pg/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 105.2 95% - 114%
    Plasma 110.4 84% - 120%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-28A (Interleukin 28A) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28A in plasma and cell culture supernatants.


    This assay employs an antibody specific for Human IL-28A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-28A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human IL-28A in normal serum with ab100567, only in serum spiked with Human IL-28A.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 機能
    Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
  • 配列類似性
    Belongs to the IL-28/IL-29 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • Cytokine ZCYTO20
    • EG330496
    • IFN-lambda-2
    • IFNL2
    • IL 28A
    • IL-28A
    • IL28A
    • IL28A_HUMAN
    • Interferon lambda 2
    • Interferon lambda-2
    • Interleukin 28A
    • Interleukin-28A
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-28A ELISA Kit (Interleukin-28A) 画像

  • Representative standard curve using ab100567

プロトコール

Human IL-28A ELISA Kit (Interleukin-28A) (ab100567) 使用論文

ab100567 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately, we do not have any cross reactivity data with ab100567 and IL-28B. Since human IL-28A does have very high homology with human IL-28B (96%) it is possible that the antibody pair in the kit is also detecting IL-28B. However, without data t...

Read More

Thank you for your patience.

Unfortunately, it looks like that neither the cross-reactivity has been determined, nor the epitope for this antibody mapped. However, as the two protein are up to 95% identical, it is very likely that the antibody...

Read More

Thank you for your inquiry.

Unfortunately, epitope mapping has not been performed for the antibodies used in the ab100567, nor has IL-28B been tested with this kit so the cross reactivity with IL-28B is not known. Therefore this information w...

Read More

Thank you for contacting us.

Unfortunately, we do not have any cross reactivity data of ab100567 with IL-28B. Since human IL-28A does have very high homology with human IL-28B (96%), it is possible that the antibody pair in the kit will also...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録