Human IL-28A ELISA Kit (Interleukin-28A) (ab100567)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-28A ELISA Kit (Interleukin-28A)
IL-28A キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 15 pg/ml -
検出範囲
13.72 pg/ml - 10000 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 105.2 95% - 114% Plasma 110.4 84% - 120% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-28A (Interleukin 28A) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28A in plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-28A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-28A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human IL-28A in normal serum with ab100567, only in serum spiked with Human IL-28A.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human IL-28A 2 vials IL-28A Microplate (12 x 8 wells) 1 unit Recombinant Human IL-28A Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. -
配列類似性
Belongs to the IL-28/IL-29 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Cytokine ZCYTO20
- IFN-lambda-2
- IFNL2
see all -
参照データベース
- Entrez Gene: 282616 Human
- Omim: 607401 Human
- SwissProt: Q8IZJ0 Human
- Unigene: 567792 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab100567 は論文での使用が確認できていません。