製品の概要

  • 製品名Human IL-23 ELISA Kit (Interleukin-23)
    IL23 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 6 4637pg/ml 72 1.6%
    B 6 3908pg/ml 36 1%
    C 6 3016pg/ml 72 2.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 9 4439pg/ml 276 6.2%
    B 9 3807pg/ml 353 9.2%
    C 9 2570pg/ml 388 15%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    156.2 pg/ml - 5000 pg/ml
  • 添加回収試験

    89 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 118 103% - 134%

  • 全工程の試験時間
    2h 45m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-23 (Interleukin-23) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-23 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

    A monoclonal antibody specific for IL-23 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-23 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-23 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-23 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-23.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 組織特異性Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • 配列類似性Belongs to the IL-6 superfamily.
  • 発生段階Expressed by newborns dendritic cells.
  • 細胞内局在Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • 別名
    • IL 23
    • IL 23A
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • Il23a
    • IL23A_HUMAN
    • IL23P19
    • Interleukin 23 alpha subunit p19
    • Interleukin-23 subunit alpha
    • Interleukin-23 subunit p19
    • JKA3 induced upon T cell activation
    • P19
    • SGRF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab64708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-23 ELISA Kit (Interleukin-23) 画像

  •  

    Representative Standard Curve using ab64708

プロトコール

Human IL-23 ELISA Kit (Interleukin-23) (ab64708) 使用論文

This product has been referenced in:
  • Yu X  et al. Intensive Cytokine induction in Pandemic H1N1 Influenza Virus Infection Accompanied by Robust Production of IL-10 and IL-6. PLoS One 6:e28680 (2011). ELISA . Read more (PubMed: 22174866) »
  • Abe T  et al. Combination use of immune complexes and a Ca2(+) channel blocker azelnidipine enhances interleukin-12 p40 secretion without T helper type 17 cytokine secretion in human monocyte-derived dendritic cells. Clin Exp Immunol 156:405-12 (2009). ELISA . Read more (PubMed: 19438591) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

The second antibody used in kit is now published. Please check datasheet of ab136544.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informat...

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Thank you for your email.

I apologise for confusion. This kit ab64708 has two mouse monoclonal antibodies in it, one is clone B-F43 and other is B-Z23. The clone B-Z23 is available for purchasing with abID 84471. The clone B-F43 is not yet ava...

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Thank you for contacting us.

The antibody ab84471 which is one of antibody in kit binds to subunit p19.

I have ask the datasheet team to add clone B-F43 in catalogue which is the second antibody in the kit. I will let you know the ca...

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Thank you for your interest in the MMP-3 and IL23 kits.

The volume of sample required for each test is 100ul per well of the assay plate.

Regarding the 100% discount, I want to be sure you understand the offer, which is also descr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"