製品の概要

  • 製品名
    Human IL-21 ELISA Kit (Interleukin-21)
    IL21 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8 5.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8 7.7%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    20 pg/ml
  • 検出範囲
    78 pg/ml - 5000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95 % - %
    Serum 71 % - %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-21 (Interleukin-21) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-21 (Interleukin-21)concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).

    IL-21 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-21 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-21 captured on the plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human IL21 monoclonal antibody 1 x 70µl
    Human IL-21 Standard lyophilized (10 ng/ml upon reconstitution) 2 vials
    Microplate coated with monoclonal antibody to Human IL-21 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究分野
  • 関連性
    A novel cytokine related to IL2 and IL15 was recently identified and designated IL21. IL21 has been found to be a powerful growth factor for naive B cells. The receptor for IL21 (IL21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL21 signaling. Both IL21R and the gc are necessary for the IL21 function. IL21 and its receptor activate JAKSTAT signaling pathway. IL21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL21 plays a role in the proliferation and maturation of NK, B and T cell populations.
  • 細胞内局在
    Secreted
  • 別名
    • Cytokine CX2
    • DN 452A22.7
    • IL 21
    • Interleukin 21
    • Interleukin 21 isoform
    • Interleukin21
    • OTTMUSP00000007838
    • Za11
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119542.

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参考文献

ab119542 has not yet been referenced specifically in any publications.

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